Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,720,897	+C	coding (420/1110 nt)	ydhH ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,720,894	1	.	C	100.0%	53.5 / NA	16	G141G (GGA→GGG)	ydhH	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/16);  total (0/16)

GCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAA  >  W3110S.gb/1720868‑1720931                            |                                      gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:1419489/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:1697400/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:1718893/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:193420/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:1990484/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:2509282/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:2908776/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:2963933/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:30700/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:311395/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:420817/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:467554/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:550162/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:780495/65‑1 (MQ=255) gCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:967449/65‑1 (MQ=255)                 cgcCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACaa  <  1:2665463/49‑1 (MQ=38)                            |                                      GCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAA  >  W3110S.gb/1720868‑1720931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑