Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,855,181	(A)7→8	coding (513/1359 nt)	ydjE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,855,174	1	.	A	97.1%	108.0 / ‑2.8	34	coding (520/1359 nt)	ydjE	predicted transporter
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (16/17);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑A‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAG  >  W3110S.gb/1855115‑1855213                                                             |                                         tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:2719668/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:2425335/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:882017/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:592018/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:1126980/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:180514/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:1624877/1‑64 (MQ=255) tGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑a‑aaa                                     >  1:1535539/1‑64 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAAGATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:2353458/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:2915885/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:2298122/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:2253195/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:174282/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:642100/65‑1 (MQ=255)           cAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATaa                           <  1:1235983/65‑1 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCAGGCCTATcgc           >  1:1231960/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:796843/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:2912944/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:2660654/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:2595306/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:2451931/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:916280/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:1123674/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:814711/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:1301804/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:804404/1‑64 (MQ=255)                            aaTACCACCCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATcgc           >  1:1732199/1‑64 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:552769/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:1019980/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:721901/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:936516/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:539184/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:2401952/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:1560015/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:153197/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:1054404/65‑1 (MQ=255)                                ccaccCAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:1034844/65‑1 (MQ=255)                                    ccAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGAt                             >  1:152981/1‑38 (MQ=38)                                    ccAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAg  >  1:2362451/1‑65 (MQ=255)                                    ccAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAg  >  1:145092/1‑65 (MQ=255)                                    ccAGCAGAAACATTATTCGCCAACTAA‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAg  >  1:1107885/1‑65 (MQ=255)                                                       ccAACT‑ATAAAAAAGCGATAACCACCACGCCTATCGCCGCAg      <  1:1169472/42‑1 (MQ=38)                                                             |                                         TGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACT‑A‑AAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAG  >  W3110S.gb/1855115‑1855213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑