Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,919,937	+C	coding (714/2634 nt)	yebT →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,919,937	1	.	C	100.0%	15.8 / NA	6	V238V (GTG→GTC)	yebT	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/6);  total (0/6)

CGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGG  >  W3110S.gb/1919898‑1919969                                         |                                 cGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt           <  1:2578982/64‑1 (MQ=255) cGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt           <  1:2784009/64‑1 (MQ=255)  ggCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt           <  1:995447/63‑1 (MQ=255)          tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg  <  1:1275108/64‑1 (MQ=255)          tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg  <  1:1433602/64‑1 (MQ=255)          tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg  <  1:3074272/64‑1 (MQ=255)                                         |                                 CGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGG  >  W3110S.gb/1919898‑1919969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑