| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 348,961 | +G | intergenic (+165/‑275) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 348,957 | 1 | . | G | 100.0% | 12.0 / NA | 5 | intergenic (+161/‑279) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (5/0); total (5/0) | |||||||||||
TGCGCCGCTGACGTGACTTATCAGGCCTAC‑GGGGTGCACTGAACTGTAGGTCGGATAAGACG > W3110S.gb/348928‑348989 | tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1357735/1‑63 (MQ=255)tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1517262/1‑63 (MQ=255)tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1587929/1‑63 (MQ=255)tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:2248766/1‑63 (MQ=255)tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:2683416/1‑63 (MQ=255) | TGCGCCGCTGACGTGACTTATCAGGCCTAC‑GGGGTGCACTGAACTGTAGGTCGGATAAGACG > W3110S.gb/348928‑348989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |