| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 746,141 | 0 | A | T | 67.7% | 36.7 / 30.3 | 34 | E185D (GAA→GAT) | ybgL | predicted lactam utilization protein |
| Reads supporting (aligned to +/- strand): ref base A (0/11); new base T (0/23); total (0/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAACGCTGGAGATGGT > W3110S.gb/746094‑746170 | tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:2840481/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:1149680/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:1223972/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:1350799/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:1573669/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:1761977/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:2843851/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:2862416/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:2067026/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:626538/65‑1 (MQ=255)tCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAAc < 1:435422/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACTCTGGAGATGGt < 1:953034/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:717234/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:3110377/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:368583/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:674421/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2497305/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:827347/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:879088/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:987600/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2832101/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2650488/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1048015/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2288964/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2118555/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2094827/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:2090484/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1961747/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1919409/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1811364/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1672588/65‑1 (MQ=255) cgAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1055459/65‑1 (MQ=255) gAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:1917267/64‑1 (MQ=255) tgaAAACGAAGATCGGACGCTGGCGCAAACGCTGGAGATGGt < 1:377565/42‑1 (MQ=37) | TCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCAAACGCTGGAGATGGT > W3110S.gb/746094‑746170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |