Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb978,8190AT61.2% 37.1 / 58.1 49D691V (GAC→GTC) mukBfused chromosome partitioning proteins
Reads supporting (aligned to +/- strand):  ref base A (0/19);  new base T (0/30);  total (0/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATC  >  W3110S.gb/978765‑978883
                                                      |                                                                
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:2351309/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:295049/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:1937798/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:2062390/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:1764502/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:529772/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:1436522/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:738169/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:1161387/65‑1 (MQ=255)
gTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:116137/65‑1 (MQ=255)
 tCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:2527048/64‑1 (MQ=255)
 tCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGc                                                        <  1:2906017/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCGGTACTTCTCAgc                              <  1:2773784/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2669220/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2703565/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2723472/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1099838/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2353801/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:449667/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:3061059/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:39777/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2370162/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:538452/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:60202/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:713496/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:773187/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:797892/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:822816/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:829805/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:933657/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1956839/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2294391/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2333134/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1930434/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1402499/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1403841/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1722103/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2219510/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2206708/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1663441/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1434466/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2196961/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2026171/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2758032/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1309283/64‑1 (MQ=255)
                           gtggtgTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:235518/64‑1 (MQ=255)
                                 tgctgcTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:1477702/58‑1 (MQ=255)
                                   ctgctgTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:2068168/56‑1 (MQ=255)
                                                   aTGTCGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAgc                              <  1:326175/40‑1 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:1419382/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:1138620/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:758780/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:141906/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2019683/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:3016280/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:292542/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2925017/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2049701/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2894524/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2718451/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2548719/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2542453/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2292134/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2489179/1‑65 (MQ=255)
                                                      acgacgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATc  >  1:2442367/1‑65 (MQ=255)
                                                      |                                                                
GTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATC  >  W3110S.gb/978765‑978883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: