| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,974,376 | 0 | C | T | 66.7% | 29.9 / 25.1 | 30 | L10L (CTG→CTA) | tar | methyl‑accepting chemotaxis protein II |
| Reads supporting (aligned to +/- strand): ref base C (2/8); new base T (0/20); total (2/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCT > W3110S.gb/1974349‑1974434 | tGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCAcc > 1:473879/1‑65 (MQ=255)tGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCAcc > 1:1665770/1‑65 (MQ=255) aGCTCCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:691633/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:1097280/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:1882024/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:677992/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:703213/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:1476131/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:715795/65‑1 (MQ=255) aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACaa < 1:1187017/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:789959/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:100158/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:590022/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:922826/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:46246/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:388730/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:2864919/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:285744/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:2765305/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:2647858/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:2468738/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:221959/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1938907/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1789820/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1605696/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:138582/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1376435/65‑1 (MQ=255) tACCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1368903/65‑1 (MQ=255) aCCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:2011414/64‑1 (MQ=255) aCCAATAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGAtct < 1:1813047/64‑1 (MQ=255) | TGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCT > W3110S.gb/1974349‑1974434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |