| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,101,391 | 0 | G | . | 57.6% | ‑5.4 / 68.1 | 33 | coding (360/1167 nt) | ugd | UDP‑glucose 6‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base . (0/19); total (7/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACC > W3110S.gb/2101344‑2101412 | atatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttgatttgat > 1:1853941/1‑65 (MQ=255)atatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttgatttgat > 1:2901887/1‑65 (MQ=255)atatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttgatttgat > 1:2514015/1‑65 (MQ=255) atTTTCAGTTCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:665793/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTtgatg > 1:1132191/1‑64 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTtgatg > 1:991228/1‑64 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTtgatg > 1:549095/1‑64 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:1410350/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:970556/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:495906/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:385863/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:372176/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2656975/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2591189/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2373331/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:1688391/65‑1 (MQ=255) atTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2310384/65‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:1546718/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:961881/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:783813/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2077070/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2795250/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2770403/64‑1 (MQ=255) ttttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2322940/64‑1 (MQ=255) gTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAAC‑GGAACCGTTGATTTGATGAc < 1:2467560/58‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:1909978/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:442960/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:1838469/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:1643627/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:961610/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:2598009/37‑1 (MQ=255) gCTGCGGTAAAACCAACGGGAACCGTTGATTtgatga < 1:1561801/37‑1 (MQ=255) cGGTAAAACCAACGGGAACCGTTGATTTGATGAcc > 1:2932971/1‑35 (MQ=255) | ATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACC > W3110S.gb/2101344‑2101412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |