| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,432,943 | 1 | . | T | 57.7% | 43.9 / 40.4 | 26 | coding (295/783 nt) | argT | lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (11/0); new base T (15/0); total (26/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGA > W3110S.gb/2432892‑2433005 | aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1038949/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:700933/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:586964/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2882015/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2726276/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2626396/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2362863/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2315041/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2214036/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1297822/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1938929/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1761005/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1789586/1‑64 (MQ=255)aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:1961192/1‑64 (MQ=255)aaCGAGAATATGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTTATCGGTaa > 1:2124836/1‑64 (MQ=25) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTa > 1:2895820/1‑39 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAg > 1:101725/1‑61 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGt > 1:1834120/1‑64 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2324755/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2597055/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:1815220/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:1879376/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2221109/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2162151/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2081100/1‑65 (MQ=255) ttGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTc > 1:2022830/1‑65 (MQ=255) g‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGa < 1:2569153/63‑1 (MQ=255) | AACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACG‑TTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGA > W3110S.gb/2432892‑2433005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |