Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,650,789	0	A	G	63.2%	13.1 / 15.6	19	V52A (GTG→GCG)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (7/0);  new base G (12/0);  total (19/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTC  >  W3110S.gb/2650745‑2650841                                             |                                                     gccccTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTc                                  >  1:2904141/4‑65 (MQ=255) gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACt                                   >  1:2007649/1‑64 (MQ=255) gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTc                                  >  1:149490/1‑65 (MQ=255) gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTc                                  >  1:1982371/1‑65 (MQ=255) gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTc                                  >  1:236822/1‑65 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:1211094/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2962678/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2844705/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2798300/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2458767/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2226363/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2128948/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:2111003/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:1651806/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:1397485/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:1358551/1‑64 (MQ=255)                         ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt           >  1:1169863/1‑64 (MQ=255)                                   tctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTc  >  1:2964016/1‑63 (MQ=255)                                   tctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTc  >  1:676188/1‑63 (MQ=255)                                             |                                                     GCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTC  >  W3110S.gb/2650745‑2650841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑