Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,585,0350TC61.1% 23.6 / 38.7 36E100E (GAA→GAG) yihIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/4);  new base C (0/22);  total (10/26)
Fisher's exact test for biased strand distribution p-value = 3.94e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCGGT  >  W3110S.gb/3584991‑3585080
                                            |                                             
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCTGt                             <  1:395731/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:876176/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:3001489/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2967100/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2944681/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2679925/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2666874/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2653027/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2578125/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:248776/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1039438/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:3008943/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:2415530/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:442642/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:515780/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:649392/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1938213/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1819941/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1443793/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1411876/63‑1 (MQ=255)
aGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:1271717/63‑1 (MQ=255)
 gACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAACTCCAACTCCGCCTGCGGt                             <  1:300509/62‑1 (MQ=255)
          gcagcGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAATCATAGGt                 <  1:809227/65‑1 (MQ=255)
          gcagcGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGt                 <  1:1569207/65‑1 (MQ=255)
          gcagcGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGt                 <  1:141032/65‑1 (MQ=255)
          gcagcGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGt                 <  1:1176540/65‑1 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:836554/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:2443934/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:2429425/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:2349071/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:2061224/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:2039322/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:1510364/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCgg   >  1:1160494/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCACACTCTTCgg   >  1:2237364/1‑65 (MQ=255)
                        cGCTCATCCGTTTCCAGTAATT‑CAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCGGt  >  1:2322322/1‑65 (MQ=255)
                                            |                                             
AGACGTTCCAGCAGCGCATCCAGACGCTCATCCGTTTCCAGTAATTCCAACTCCGCCTGCGGTGAAAGCATAGGTTTCTCACTCTTCGGT  >  W3110S.gb/3584991‑3585080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑