| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,069,539 | 1 | . | G | 59.1% | 42.1 / 29.0 | 22 | coding (442/1974 nt) | glgX | glycogen debranching enzyme |
| Reads supporting (aligned to +/- strand): ref base . (4/5); new base G (13/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGA > W3110S.gb/4069477‑4069561 | cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2984321/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2247470/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2331182/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2453009/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2498991/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2518429/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2555231/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2589352/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2814807/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2933434/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1076895/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2993217/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:3072713/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:3097360/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:533223/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:584861/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:678245/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:72549/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:772649/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:937597/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1632356/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1047348/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:120622/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1227259/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1620769/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2222459/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1753837/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:181692/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1850700/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:1995141/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑gg > 1:2104752/1‑65 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑g > 1:573534/1‑64 (MQ=255)cGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑g > 1:1239606/1‑64 (MQ=255) ttGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTa > 1:2374385/1‑64 (MQ=255) ttGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTa > 1:1480963/1‑64 (MQ=255) ttGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTa > 1:167819/1‑64 (MQ=255) ttGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTa > 1:262864/1‑64 (MQ=255) gATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGa < 1:829918/65‑1 (MQ=255) gATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGa < 1:1281134/65‑1 (MQ=255) gATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGa < 1:2297401/65‑1 (MQ=255) gATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGG‑GGGGCAGCACCATCATTTATGa < 1:3089678/65‑1 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:2329147/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:406513/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:667166/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:862816/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:577733/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:568006/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:1439980/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:1585033/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:2871039/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:2000866/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:2711922/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:1797708/1‑65 (MQ=255) aTCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGGCAGCACCATCATTTATGa > 1:2477337/1‑65 (MQ=255) tGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGa < 1:2352681/57‑1 (MQ=255) | CGAAATGCGTAGTGGTGGTTGATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGT‑GGGGCAGCACCATCATTTATGA > W3110S.gb/4069477‑4069561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |