Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,512,230	A→G	W371R (TGG→CGG)	ypdG ←	predicted enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,512,230	0	A	G	100.0%	111.0 / NA	31	W371R (TGG→CGG)	ypdG	predicted enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

CTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGG  >  W3110S.gb/2512170‑2512280                                                             |                                                   cTGCCCCCAGCGCGCTCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGAt                                                 >  1:2269966/1‑64 (MQ=255) cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGAt                                                 >  1:1744039/1‑64 (MQ=255)                     gCCATATAGACGCCCAGGTTAGTGACCAGCGGTCATGCCCGGATAGCGGACTCCGGGAACCATTg                            >  1:2799789/1‑65 (MQ=255)                     gCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                            >  1:943757/1‑65 (MQ=255)                     gCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                            >  1:2741636/1‑65 (MQ=255)                     gCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                            >  1:2738021/1‑65 (MQ=255)                     gCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                            >  1:1589506/1‑65 (MQ=255)                               cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                  >  1:2181844/1‑65 (MQ=255)                               cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                  >  1:1939893/1‑65 (MQ=255)                               cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                  >  1:1891644/1‑65 (MQ=255)                               cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                  >  1:1263309/1‑65 (MQ=255)                               cGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGccc                  >  1:1291657/1‑65 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:1825711/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:1194064/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:2933783/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:2738746/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:1471676/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:2588536/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:23042/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:150321/37‑1 (MQ=255)                                        tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:1638705/37‑1 (MQ=255)                                         aGTGACCAGCGGCCATGCCCGGATAGCGGACTCCgg                                     <  1:1472705/36‑1 (MQ=255)                                            gACCAGCGTCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:254166/64‑1 (MQ=255)                                            gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:2352509/64‑1 (MQ=255)                                            gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:2573034/64‑1 (MQ=255)                                            gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:1140405/64‑1 (MQ=255)                                            gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:3065969/64‑1 (MQ=255)                                            gACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg      <  1:816648/64‑1 (MQ=255)                                                 gCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg  >  1:2089999/1‑63 (MQ=255)                                                 gCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg  >  1:596451/1‑63 (MQ=255)                                                 gCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg  >  1:1760433/1‑63 (MQ=255)                                                             |                                                   CTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGG  >  W3110S.gb/2512170‑2512280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑