Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,550,560	A→G	C166R (TGC→CGC)	yfeT ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,550,560	0	A	G	100.0%	73.9 / NA	22	C166R (TGC→CGC)	yfeT	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/0);  total (22/0)

GCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGAT  >  W3110S.gb/2550507‑2550571                                                      |            gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGc          >  1:1775164/1‑57 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCTACGCGAt  >  1:905193/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGa   >  1:1830985/1‑64 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:21238/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:912235/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:852389/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:642335/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:3033632/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2939413/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2927438/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2676234/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2672511/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2203451/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1052707/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2104738/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:2053003/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:197596/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1791703/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1428424/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1323928/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1219355/1‑65 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGAt  >  1:1202409/1‑65 (MQ=255)                                                      |            GCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGAT  >  W3110S.gb/2550507‑2550571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑