Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,559,548	T→C	I293T (ATT→ACT)	hemF →	coproporphyrinogen III oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,559,548	0	T	C	100.0%	32.7 / NA	10	I293T (ATT→ACT)	hemF	coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/0);  total (10/0)

CGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCG  >  W3110S.gb/2559487‑2559596                                                              |                                                 cGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTaa                                               >  1:269457/1‑65 (MQ=255) cGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTaa                                               >  1:718039/1‑65 (MQ=255) cGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTaa                                               >  1:889940/1‑65 (MQ=255)                                          aGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTggg                                 >  1:2687609/1‑38 (MQ=255)                                          aGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCa      >  1:2607543/1‑65 (MQ=255)                                          aGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCa      >  1:832383/1‑65 (MQ=255)                                              gcgTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCg  >  1:1131070/1‑65 (MQ=255)                                              gcgTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCg  >  1:2029238/1‑65 (MQ=255)                                              gcgTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCg  >  1:2264318/1‑65 (MQ=255)                                              gcgTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCg  >  1:2603549/1‑65 (MQ=255)                                                              |                                                 CGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCG  >  W3110S.gb/2559487‑2559596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑