Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,560,022	G→A	P203S (CCT→TCT)	yfeG ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,560,022	0	G	A	100.0%	138.4 / NA	44	P203S (CCT→TCT)	yfeG	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/11);  total (33/11)

TTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACA  >  W3110S.gb/2559986‑2560074                                     |                                                     ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:901840/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:870115/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1333970/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1357888/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:3092359/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:2125462/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:2634910/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:2500336/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1716435/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1720025/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1849574/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa                          >  1:1827094/1‑65 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCa                           >  1:2159714/1‑64 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTAGCaa                          >  1:2371726/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt                     >  1:469770/1‑64 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:816429/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:2549916/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:2485811/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:558520/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:517557/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:373068/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:477281/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:2174965/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:2055962/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1764443/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1695988/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1662856/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1582787/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1499608/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1483575/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1288340/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc                    >  1:1188740/1‑65 (MQ=255)       aaTTATCCCCCAGCACTTTTCGCACTCCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt                     >  1:312797/1‑64 (MQ=255)                           cGCACTGCAGACTGATTGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:351627/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:1135749/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:738077/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:925643/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2901787/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2751273/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2683161/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2520552/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:24494/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2179917/63‑1 (MQ=255)                           cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa  <  1:2073783/63‑1 (MQ=255)                                     |                                                     TTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACA  >  W3110S.gb/2559986‑2560074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑