Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,620,788	A→G	E312E (GAA→GAG)	purM →	phosphoribosylaminoimidazole synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,620,788	0	A	G	100.0%	16.7 / NA	6	E312E (GAA→GAG)	purM	phosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/4);  total (2/4)

GCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAA  >  W3110S.gb/2620754‑2620840                                   |                                                     gcgTCGGGATGATTATTGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGcc                        >  1:2990935/1‑65 (MQ=255)          tgatTATTGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGaa               >  1:1528847/1‑65 (MQ=255)                       gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa  <  1:1789691/65‑1 (MQ=255)                       gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa  <  1:616261/65‑1 (MQ=255)                       gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa  <  1:6229/65‑1 (MQ=255)                       gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa  <  1:734595/65‑1 (MQ=255)                                   |                                                     GCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAA  >  W3110S.gb/2620754‑2620840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑