Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,632,142	C→T	M195I (ATG→ATA)	guaB ←	IMP dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,632,142	0	C	T	91.7%	30.8 / ‑3.4	12	M195I (ATG→ATA)	guaB	IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (3/8);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTT  >  W3110S.gb/2632090‑2632196                                                     |                                                       cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTTATTATGCCGATCAg                                             <  1:1925284/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:1619789/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:2096860/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:2177328/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:2224921/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:512126/64‑1 (MQ=255) cTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:590012/64‑1 (MQ=255)  tttACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAg                                             <  1:2478092/63‑1 (MQ=255)              cGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAg                                             <  1:2717787/51‑1 (MQ=255)                             tttCTGGAAGTCTTTCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCtt                >  1:113320/1‑65 (MQ=255)                             tttCTGGAAGTCTTTCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCtt                >  1:2606219/1‑65 (MQ=255)                                           tCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGtttt  >  1:2101859/1‑65 (MQ=255)                                                     |                                                       CTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTT  >  W3110S.gb/2632090‑2632196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑