Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,650,810	G→A	A45V (GCG→GTG)	yfhM ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,650,810	0	G	A	85.7%	32.4 / ‑0.4	14	A45V (GCG→GTG)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (12/0);  total (14/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCG  >  W3110S.gb/2650769‑2650873                                          |                                                                ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:1211094/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2962678/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2844705/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2798300/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2458767/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2226363/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2128948/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:2111003/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:1651806/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:1397485/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:1358551/1‑64 (MQ=255) ccAGTTTTTGTCGCTCCGGCGCGGAAAGCTTTGCTGAACTCACGTTTTCTGAAGAGGCCGCttt                                           >  1:1169863/1‑64 (MQ=255)           tctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTc                                  >  1:2964016/1‑63 (MQ=255)           tctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTc                                  >  1:676188/1‑63 (MQ=255)                                         cgcgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg  <  1:1961636/65‑1 (MQ=255)                                         cgcgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg  <  1:1400366/65‑1 (MQ=255)                                         cgcgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg  <  1:2798098/65‑1 (MQ=255)                                         cgcgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg  <  1:1385156/65‑1 (MQ=255)                                         cgcgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg  <  1:413849/65‑1 (MQ=255)                                          |                                                                CCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCG  >  W3110S.gb/2650769‑2650873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑