Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,732,756	T→A	D25V (GAC→GTC)	clpB ←	protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,732,756	0	T	A	100.0%	49.8 / NA	17	D25V (GAC→GTC)	clpB	protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/5);  total (12/5)

AACCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCA  >  W3110S.gb/2732697‑2732770                                                            |               aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTg            >  1:1666139/1‑64 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTg            >  1:701038/1‑64 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:844215/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:1535136/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:1704393/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:2421805/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:2456645/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:3089948/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:2486095/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:2491791/1‑65 (MQ=255) aaCCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:3006337/1‑65 (MQ=255) aaCCACCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGc           >  1:1177523/1‑65 (MQ=255)            tGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCa  <  1:3084698/63‑1 (MQ=255)            tGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCa  <  1:2463706/63‑1 (MQ=255)            tGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCa  <  1:2146358/63‑1 (MQ=255)            tGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCa  <  1:784802/63‑1 (MQ=255)            tGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCa  <  1:1126957/63‑1 (MQ=255)                                                            |               AACCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCA  >  W3110S.gb/2732697‑2732770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑