Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	505,179	C→T	L348L (CTG→TTG)	ushA →	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	505,179	0	C	T	100.0%	125.6 / NA	39	L348L (CTG→TTG)	ushA	bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

TGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTG  >  W3110S.gb/505157‑505223                       |                                             tGAAAACCAGCTAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1845121/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1202801/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:550893/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:54288/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:511007/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:364340/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:2959379/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:2357056/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:2019961/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1854439/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1566873/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1564651/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1472486/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1425790/65‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAg    <  1:1187016/65‑1 (MQ=255)     aaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg  <  1:351608/63‑1 (MQ=255)     aaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg  <  1:2520710/63‑1 (MQ=255)     aaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg  <  1:520983/63‑1 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg           >  1:918155/1‑40 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1236737/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:811541/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:876210/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1270921/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1298754/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:892253/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2746515/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:3061813/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1353234/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2933582/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2747964/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2641723/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2612739/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:249369/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1414217/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2330707/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:2192180/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1898596/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1831255/1‑45 (MQ=255)                   ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGa      >  1:1740350/1‑45 (MQ=255)                       |                                             TGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTG  >  W3110S.gb/505157‑505223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑