Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,770,406	T→C	intergenic (+135/+90)	yfjT → / ← yfjU	hypothetical protein/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,770,406	0	T	C	100.0%	58.5 / NA	17	intergenic (+135/+90)	yfjT/yfjU	hypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/2);  total (15/2)

ACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCT  >  W3110S.gb/2770358‑2770460                                                 |                                                       aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGc                                          >  1:1300483/1‑63 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:1780336/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:933233/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:792193/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:415979/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:2839858/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:261815/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:2090475/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:1813128/1‑64 (MQ=255) aCGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCt                                         >  1:1795730/1‑64 (MQ=255)        ttCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGTAAGCt                                  <  1:321768/64‑1 (MQ=255)           gAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGTAAGCt                                  <  1:561654/61‑1 (MQ=255)                                       tCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCt  >  1:2594356/1‑65 (MQ=255)                                       tCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCt  >  1:1961007/1‑65 (MQ=255)                                       tCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCt  >  1:1874576/1‑65 (MQ=255)                                       tCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCt  >  1:183400/1‑65 (MQ=255)                                       tCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCAGTGTTAAAACTCCt  >  1:3045942/1‑65 (MQ=255)                                                 |                                                       ACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCT  >  W3110S.gb/2770358‑2770460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑