Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,841,701	C→T	L134L (CTG→CTA)	hycH ←	protein required for maturation of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,841,701	0	C	T	100.0%	161.0 / NA	48	L134L (CTG→CTA)	hycH	protein required for maturation of hydrogenase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/18);  total (30/18)

CCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTG  >  W3110S.gb/2841650‑2841736                                                    |                                    ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:886744/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:4514/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:474310/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:57258/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:290176/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2694301/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2682350/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2367601/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2353425/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2211654/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:717993/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:2096499/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:782310/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:868511/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:1509658/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:1482572/64‑1 (MQ=255) ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc                         <  1:140344/64‑1 (MQ=255)    tcatcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCa                     <  1:2996417/65‑1 (MQ=255)                    tATTGCCAACACAGAGTAAATCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTc     >  1:36268/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGAt              >  1:2558921/1‑53 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg  >  1:1195750/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg  >  1:1368714/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg  >  1:101753/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg  >  1:2619873/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg  >  1:3002878/1‑65 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt   >  1:2426666/1‑64 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt   >  1:2329734/1‑64 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt   >  1:2031953/1‑64 (MQ=255)                       tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTAACCATCAAATAGATGGCGTTTTCCtg  >  1:119670/1‑65 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2660218/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:584676/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:607178/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:672311/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:854191/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:749948/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:471942/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:3010531/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2960155/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2842110/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2618609/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2317784/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:2134655/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:210258/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:1963578/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:1746156/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:1149627/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa                 >  1:1131652/1‑45 (MQ=255)                            acacAGAGTAAAACGTCAGTCACGTAGACTCCTCACCATCAAATa                 >  1:873043/1‑45 (MQ=255)                                                    |                                    CCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTG  >  W3110S.gb/2841650‑2841736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑