Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,894,340	C→T	W32* (TGG→TGA)	ygcQ ←	predicted flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,894,340	0	C	T	100.0%	149.7 / NA	45	W32* (TGG→TGA)	ygcQ	predicted flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

ACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGC  >  W3110S.gb/2894282‑2894386                                                           |                                               aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCATTg                                           <  1:2281330/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:1050739/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:1123201/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:903389/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:1327984/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:1405966/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:2350635/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:1898756/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:151150/64‑1 (MQ=255) aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                           <  1:2836156/64‑1 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTTAGTGCGCCAGGGTAc                                >  1:1083761/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:486953/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:3101183/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:3056563/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:2909956/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:522093/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:2668914/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:580248/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:853666/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:2300134/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:94087/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:2016166/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:10927/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:1414826/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:1453436/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:1615649/1‑65 (MQ=255)           caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc                                >  1:1762513/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:272006/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:2864721/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:2869784/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:235144/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:2983646/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:1595566/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:1595422/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:1574718/1‑65 (MQ=255)                               ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc            >  1:1581299/1‑65 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:2282885/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:2006506/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:726077/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:2962762/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:292158/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:1762115/64‑1 (MQ=255)                                          ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:2733347/64‑1 (MQ=255)                                           gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:1184776/63‑1 (MQ=255)                                             tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc  <  1:470827/61‑1 (MQ=255)                                                           |                                               ACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGC  >  W3110S.gb/2894282‑2894386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑