Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,927,326	G→A	V148M (GTG→ATG)	sdaC →	predicted serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,927,326	0	G	A	100.0%	221.6 / NA	64	V148M (GTG→ATG)	sdaC	predicted serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/51);  total (13/51)

ATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATT  >  W3110S.gb/2927284‑2927385                                           |                                                            aTGACGCCACCTCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:133823/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1806879/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2368079/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2232373/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2913622/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:887679/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1364167/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2019410/65‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGGCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1434732/65‑1 (MQ=255)     cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                                    >  1:690461/1‑64 (MQ=255)     cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                                    >  1:555594/1‑64 (MQ=255)      gCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:658680/60‑1 (MQ=255)                cgTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                  >  1:779613/1‑39 (MQ=255)                cgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                        >  1:1479825/1‑65 (MQ=255)                cgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                        >  1:2059510/1‑65 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1925084/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2960305/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2562387/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2939182/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1980407/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:24580/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2972701/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:304722/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1868373/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1755703/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:401451/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:527656/54‑1 (MQ=255)                    cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:939154/54‑1 (MQ=255)                     gATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1034053/53‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCCGATGATCGTTAAAGCGa          <  1:234083/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:899370/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1002454/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:2882559/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:2601096/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:978375/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1539371/65‑1 (MQ=255)                              gCTGATCCTGATCATGGGTATGACGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:591677/65‑1 (MQ=255)                               ctgatcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1870055/64‑1 (MQ=255)                                  atgctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:396981/4‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:12387/1‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:289257/1‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1292758/1‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1971752/1‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:819489/1‑65 (MQ=255)                                  atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1561018/1‑65 (MQ=255)                                  atcctgatcATAGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1467884/1‑65 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGtttt  <  1:6419/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1459749/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1381088/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:80564/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:653382/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1292693/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2488006/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1087563/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:536600/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:466883/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:40333/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1911946/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1955509/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2934717/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:199945/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2487858/64‑1 (MQ=255)                                       gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2694027/64‑1 (MQ=255)                                        atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1670499/63‑1 (MQ=255)                                           |                                                            ATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATT  >  W3110S.gb/2927284‑2927385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑