Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,960,928	T→C	K53E (AAG→GAG)	recC ←	exonuclease V (RecBCD complex), gamma chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,960,928	0	T	C	100.0%	132.2 / NA	36	K53E (AAG→GAG)	recC	exonuclease V (RecBCD complex), gamma chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/9);  total (27/9)

GCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT  >  W3110S.gb/2960884‑2960989                                             |                                                              gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:104681/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:906547/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:739889/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:662357/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:417288/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:413069/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:354076/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:352224/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:350521/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:3003206/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:2800923/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:2786603/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:2486990/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:2398700/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:1736129/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:1039910/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:1365289/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:1573261/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:1870778/1‑65 (MQ=255) gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa                                           >  1:2272959/1‑65 (MQ=255)       tGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACt                                     <  1:2841948/65‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:1562492/64‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:2119193/64‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:1369553/64‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:1404322/64‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:1555927/64‑1 (MQ=255)           aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:2591026/64‑1 (MQ=255)           aGCGGAAAATCAATGTCTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg                                  <  1:3095469/64‑1 (MQ=255)                                gCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAg            <  1:2531425/65‑1 (MQ=255)                                         aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:2033013/1‑65 (MQ=255)                                         aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:52501/1‑65 (MQ=255)                                         aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc   >  1:1884562/1‑65 (MQ=255)                                          aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:234876/1‑65 (MQ=255)                                          aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:625094/1‑65 (MQ=255)                                          aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:714698/1‑65 (MQ=255)                                          aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct  >  1:2975248/1‑65 (MQ=255)                                             |                                                              GCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT  >  W3110S.gb/2960884‑2960989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑