| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,968,686 | A→G | I123M (ATA→ATG) | mutH → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,968,686 | 0 | A | G | 95.5% | 70.1 / ‑3.6 | 22 | I123M (ATA→ATG) | mutH | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (21/0); total (22/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGG > W3110S.gb/2968632‑2968733 | ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGaa > 1:1147933/1‑64 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGaa > 1:1591005/1‑64 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:99861/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1124323/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:98741/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:492505/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:354012/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:2502319/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1964618/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1917364/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1822490/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1792484/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1667429/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1593336/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:137213/1‑65 (MQ=255)ggTGACCTGGGAAACCAGCCACGTGCCCCACAAACTCAAACGCGTACTGTTGATGCCGGTTGAAg > 1:1532957/1‑65 (MQ=255) ccTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAg > 1:191449/1‑65 (MQ=255) ccTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAg > 1:2089795/1‑65 (MQ=255) ccTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAg > 1:1664250/1‑65 (MQ=255) ccTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAg > 1:489645/1‑65 (MQ=255) ccTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAg > 1:1214453/1‑65 (MQ=255) aaaCGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTgg > 1:2091081/1‑65 (MQ=255) | GGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGG > W3110S.gb/2968632‑2968733 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |