Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,971,401 | A→T | V373E (GTG→GAG) | ygeD ← | predicted inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,971,401 | 0 | A | T | 92.3% | 28.9 / ‑5.4 | 13 | V373E (GTG→GAG) | ygeD | predicted inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (3/9); minor base G (0/1); total (3/10) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCG > W3110S.gb/2971347‑2971431 | cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1212420/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1244929/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1715643/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:182802/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:1838038/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:389680/65‑1 (MQ=255) cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:983197/65‑1 (MQ=255) caGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGc < 1:2459252/65‑1 (MQ=255) ccAGCGCAAACAGCGCACCGCAGCCAATGCCAATGGGCTCGCCCGGGATGCCTATCATTACCGcc > 1:1808356/1‑65 (MQ=255) ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGcc > 1:220746/1‑65 (MQ=255) ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGcc > 1:560786/1‑65 (MQ=255) cgcAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCg < 1:721767/65‑1 (MQ=255) aatgGGCGCGACCGGGATGCCTATCATTACCGCCAGCg < 1:1305611/38‑1 (MQ=255) | CACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCG > W3110S.gb/2971347‑2971431 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |