Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,971,401 A→T V373E (GTG→GAG)  ygeD ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,971,4010AT92.3% 28.9 / ‑5.4 13V373E (GTG→GAG) ygeDpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (3/9);  minor base G (0/1);  total (3/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCG  >  W3110S.gb/2971347‑2971431
                                                      |                              
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:1212420/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:1244929/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:1715643/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:182802/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:1838038/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:389680/65‑1 (MQ=255)
cacaGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt                      <  1:983197/65‑1 (MQ=255)
  caGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGc                    <  1:2459252/65‑1 (MQ=255)
                ccAGCGCAAACAGCGCACCGCAGCCAATGCCAATGGGCTCGCCCGGGATGCCTATCATTACCGcc      >  1:1808356/1‑65 (MQ=255)
                ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGcc      >  1:220746/1‑65 (MQ=255)
                ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGcc      >  1:560786/1‑65 (MQ=255)
                    cgcAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCg  <  1:721767/65‑1 (MQ=255)
                                               aatgGGCGCGACCGGGATGCCTATCATTACCGCCAGCg  <  1:1305611/38‑1 (MQ=255)
                                                      |                              
CACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCG  >  W3110S.gb/2971347‑2971431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: