Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,971,531	G→T	L330I (CTA→ATA)	ygeD ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,971,531	0	G	T	100.0%	50.5 / NA	18	L330I (CTA→ATA)	ygeD	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/18);  total (0/18)

GCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGA  >  W3110S.gb/2971469‑2971542                                                               |            gCCAAGGTTTTGTACTGCAATCGCATTCCCCG‑CCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:2784738/64‑1 (MQ=255) gCCAAGGTTTTGTACTGCAATCGCATTCCCC‑CCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:2665714/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGGCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:3092653/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGGCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:3089300/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:789535/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:1584497/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:1783730/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:2343394/64‑1 (MQ=255)  ccAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:3047170/64‑1 (MQ=255)    aaGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCa           <  1:1411458/62‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCAttta  <  1:757804/65‑3 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:2698674/65‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:2924555/65‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:2813635/65‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:11448/65‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:227640/65‑1 (MQ=255)          ttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:1242543/65‑1 (MQ=255)            gTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGa  <  1:2641856/63‑1 (MQ=255)                                                               |            GCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGA  >  W3110S.gb/2971469‑2971542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑