Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,976,020	C→T	L256F (CTC→TTC)	galR →	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,976,020	0	C	T	100.0%	33.5 / NA	12	L256F (CTC→TTC)	galR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/11);  total (1/11)

ATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGG  >  W3110S.gb/2975985‑2976047                                    |                            ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:1194113/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:1497088/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:1931061/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:2487868/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:2567702/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:2618058/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:2780530/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:378414/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:868805/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTg           <  1:934238/54‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCgg  >  1:1176781/1‑63 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTAGTg           <  1:6760/54‑1 (MQ=255)                                    |                            ATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGG  >  W3110S.gb/2975985‑2976047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑