Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,065,306	A→G	V175A (GTT→GCT)	ygfI ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,065,306	0	A	G	100.0%	79.1 / NA	23	V175A (GTT→GCT)	ygfI	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/0);  total (23/0)

TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATA  >  W3110S.gb/3065246‑3065344                                                             |                                       tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcgag                                    <  1:2138495/65‑1 (MQ=255) tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcgag                                    <  1:327464/65‑1 (MQ=255) tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcgag                                    <  1:2444097/65‑1 (MQ=255)   tAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcgag                                    <  1:1070714/63‑1 (MQ=255)     gCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAg                                >  1:501325/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGCAAGCCTAATCGCAg                     >  1:1562175/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCa                      >  1:481521/1‑64 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:2238368/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:3040798/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:2456602/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:678965/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:2319793/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:2312570/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:1197910/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:2144988/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:52778/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:200520/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:670279/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:1465290/1‑65 (MQ=255)                tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg                     >  1:1218657/1‑65 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:880953/1‑64 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:2994978/1‑64 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:2845445/1‑64 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:1813896/1‑64 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:1174002/1‑64 (MQ=255)                                    ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa  >  1:109319/1‑64 (MQ=255)                                    ttgTCGCTATTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTAACTGATa  >  1:2104579/1‑64 (MQ=255)                                                             |                                       TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATA  >  W3110S.gb/3065246‑3065344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑