| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,179,239 | C→T | L55L (CTG→TTG) | ygiC → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,179,239 | 0 | C | T | 85.7% | 12.7 / ‑3.1 | 7 | L55L (CTG→TTG) | ygiC | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (5/1); total (5/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAC > W3110S.gb/3179206‑3179297 | cAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAATTGCACCAGATGTGCCTGAAAGTGGTGGaaaa < 1:1920673/65‑1 (MQ=255) aGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGc < 1:2631063/64‑1 (MQ=255) ccgAATTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 1:1159284/1‑64 (MQ=255) ccgAATTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 1:2213037/1‑64 (MQ=255) ccgAATTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 1:2668548/1‑64 (MQ=255) ccgAATTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 1:2794653/1‑64 (MQ=255) ccgAATTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 1:2852054/1‑64 (MQ=255) | CAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAC > W3110S.gb/3179206‑3179297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |