Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,222,206	C→T	N306N (AAC→AAT)	ebgA →	cryptic beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,222,206	0	C	T	100.0%	111.7 / NA	35	N306N (AAC→AAT)	ebgA	cryptic beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/23);  total (12/23)

GCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGG  >  W3110S.gb/3222150‑3222262                                                         |                                                         gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1258738/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:573519/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1026017/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1587741/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1590169/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1709071/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1722586/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:2877844/1‑65 (MQ=255) gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGa                                                  >  1:1803065/1‑65 (MQ=255)                                 gccGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2822872/63‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2277635/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1925697/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2309131/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2518954/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2559202/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:3076484/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:402898/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:424313/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:861289/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2169654/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:2073471/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1900968/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1873424/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:181450/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:178051/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1697384/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:152312/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1484434/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1314390/65‑1 (MQ=255)                                 gACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1061686/65‑1 (MQ=255)                                  aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:857776/64‑1 (MQ=255)                                                 aaCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACgg  >  1:2505381/1‑65 (MQ=255)                                                 aaCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACgg  >  1:786892/1‑65 (MQ=255)                                                 aaCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACg   >  1:83287/1‑64 (MQ=255)                                                      cAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata                  <  1:1835779/44‑1 (MQ=255)                                                         |                                                         GCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGG  >  W3110S.gb/3222150‑3222262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑