Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,232,470	A→G	L111L (TTG→CTG)	ygjM ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,232,470	0	A	G	100.0%	126.3 / NA	35	L111L (TTG→CTG)	ygjM	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/3);  total (32/3)

ATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATA  >  W3110S.gb/3232409‑3232530                                                              |                                                             aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:2377135/1‑65 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:2343729/1‑65 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:2373173/1‑65 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:1566937/1‑65 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:1449940/1‑65 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                           >  1:1357374/1‑65 (MQ=255) aTGCCGAATCGCGTAGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCagaa                                                            >  1:1728748/1‑64 (MQ=255)                gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTTACACCa                                                <  1:774623/61‑1 (MQ=255)                                 ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCg                           <  1:1848375/65‑1 (MQ=255)                                 ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCg                           <  1:2979156/65‑1 (MQ=255)                                       cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:949747/1‑58 (MQ=255)                                       cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:851725/1‑58 (MQ=255)                                       cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1776746/1‑58 (MQ=255)                                       cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:3065489/1‑58 (MQ=255)                                       cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1262034/1‑58 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt                               >  1:2183362/1‑52 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:999300/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:2467953/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:3097655/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:526474/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:694138/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1169975/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:2372726/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:2043640/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1751060/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1727879/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1694545/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1555647/1‑55 (MQ=255)                                          tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc                            >  1:1519736/1‑55 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:2460915/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:2620267/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:2817400/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:2074504/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:490308/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata  >  1:722175/1‑65 (MQ=255)                                                          cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGCGTTAAACCata  >  1:1532896/1‑65 (MQ=255)                                                              |                                                             ATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATA  >  W3110S.gb/3232409‑3232530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑