Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	547,694	A→G	pseudogene (114/261 nt) pseudogene (114/1259 nt)	ylbE → ylbE →	ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	547,694	0	A	G	100.0%	62.0 / NA	18	pseudogene (114/261 nt) pseudogene (114/1259 nt)	ylbE ylbE	ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

TGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCG  >  W3110S.gb/547655‑547746                                        |                                                     tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:1155978/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:743937/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:73694/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:318656/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:2043424/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:1855683/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:1784825/65‑1 (MQ=255) tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:1105358/65‑1 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:2270872/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:2711594/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:2880641/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:1577445/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:1378434/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                 >  1:979913/1‑39 (MQ=255)       aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                  >  1:1849973/1‑38 (MQ=255)       aCCGGCTTCTTCACTTATCAGCCAACTAAACGAGGGCaa                                                 >  1:1773539/1‑39 (MQ=255)                           gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                             <  1:2085345/39‑1 (MQ=255)                            cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg  <  1:1781442/65‑1 (MQ=255)                                        |                                                     TGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCG  >  W3110S.gb/547655‑547746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑