Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,277,106	A→G	T84A (ACT→GCT)	sohA →	predicted regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,277,106	0	A	G	100.0%	214.5 / NA	58	T84A (ACT→GCT)	sohA	predicted regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/39);  total (19/39)

ATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACG  >  W3110S.gb/3277044‑3277157                                                               |                                                    aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt                                                   <  1:363308/65‑1 (MQ=255) aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt                                                   <  1:1348030/65‑1 (MQ=255) aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt                                                   <  1:1038110/65‑1 (MQ=255)  tCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt                                                   <  1:2414665/64‑1 (MQ=255)      aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc                                              >  1:2426549/1‑65 (MQ=255)        tATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc                                              >  1:806299/1‑63 (MQ=255)        tATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc                                              >  1:2823042/1‑63 (MQ=255)         aTGAATTCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:3007190/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:2591527/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:988713/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:462127/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:321326/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:291761/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:2554491/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:1113502/64‑1 (MQ=255)         aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:1726835/64‑1 (MQ=255)                cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt                                                   <  1:1844291/50‑1 (MQ=255)                    tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca                                  >  1:1934522/1‑63 (MQ=255)                    tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca                                  >  1:60114/1‑63 (MQ=255)                    tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca                                  >  1:499396/1‑63 (MQ=255)                    tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca                                  >  1:405643/1‑63 (MQ=255)                                ggATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat                                            <  1:2950116/41‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:2633305/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:2878088/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:1753502/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:2913401/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:1284179/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:1266223/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:1180761/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:611552/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:823858/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:860429/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:1698686/64‑1 (MQ=255)                                       gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg              <  1:2404053/64‑1 (MQ=255)                                          atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg          <  1:1803796/65‑1 (MQ=255)                                          atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg          <  1:1842325/65‑1 (MQ=255)                                          atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg          <  1:1131991/65‑1 (MQ=255)                                          atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg          <  1:2464799/65‑1 (MQ=255)                                          atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg          <  1:570246/65‑1 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:1205526/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:925834/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2029491/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2051970/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:59747/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2077122/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:305377/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:1716899/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2930589/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2608815/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:2832265/1‑50 (MQ=255)                                                 acaacCCGCAAAAAGCTCATCCATTCAACATTCAACAAGGAAAGAAACtt                  >  1:1459635/1‑50 (MQ=255)                                                  caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:2103825/65‑1 (MQ=255)                                                  caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:1223627/65‑1 (MQ=255)                                                  caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:582788/65‑1 (MQ=255)                                                  caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:1354807/65‑1 (MQ=255)                                                  caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:2631492/65‑1 (MQ=255)                                                   aacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:1770329/64‑1 (MQ=255)                                                   aacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg  <  1:2689567/64‑1 (MQ=255)                                                               |                                                    ATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACG  >  W3110S.gb/3277044‑3277157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑