Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,291,041	A→G	L791L (CTA→CTG)	yraJ →	predicted outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,291,041	0	A	G	100.0%	71.0 / NA	20	L791L (CTA→CTG)	yraJ	predicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/20);  total (0/20)

ACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCG  >  W3110S.gb/3290995‑3291059                                               |                   aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:2762417/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:946207/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:745963/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:526556/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:373675/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:366976/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:365618/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:293527/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:2838796/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:1199719/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:262384/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:2234496/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:1650062/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:1380267/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:1333361/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:12218/65‑1 (MQ=255) aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGACGGCATACTGTATCTCTCTGGGATATCg  <  1:2693219/65‑1 (MQ=255)  cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:2658937/64‑1 (MQ=255)  cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:1523896/64‑1 (MQ=255)  cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg  <  1:525329/64‑1 (MQ=255)                                               |                   ACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCG  >  W3110S.gb/3290995‑3291059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑