Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,343,341	T→C	I36T (ATC→ACC)	yhbN →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,343,341	0	T	C	100.0%	110.6 / NA	31	I36T (ATC→ACC)	yhbN	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/27);  total (4/27)

TCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAC  >  W3110S.gb/3343303‑3343392                                       |                                                    tCCGGCATTTGCCGTAACCGGAGGCACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1273618/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACTTTGAATCGGACCAGCAAtctc                           <  1:2417269/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1070093/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:782413/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:230032/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:2157150/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:796859/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:2136207/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1970793/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1965017/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:17661/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:872401/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1683078/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1637087/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1564833/65‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1119979/65‑1 (MQ=255)  ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:778302/64‑1 (MQ=255)              gTAACCGGAGACACTGATCAGCCGACCCACATTGaa                                           >  1:413798/1‑36 (MQ=255)              gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg               >  1:1811515/1‑64 (MQ=255)              gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg               >  1:642895/1‑64 (MQ=255)              gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg               >  1:385203/1‑64 (MQ=255)                aaCCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAtctc                           <  1:1923690/50‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:1105245/65‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:760142/65‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:3079423/65‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:3058984/65‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:2445177/65‑1 (MQ=255)                          acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:2138355/65‑1 (MQ=255)                           cTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGttcc  <  1:981246/64‑3 (MQ=255)                           cTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:2505074/64‑1 (MQ=255)                           cTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc  <  1:1761958/64‑1 (MQ=255)                                       |                                                    TCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAC  >  W3110S.gb/3343303‑3343392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑