Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,348,920	T→A	L205H (CTC→CAC)	yrbL →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,348,920	0	T	A	100.0%	31.7 / NA	12	L205H (CTC→CAC)	yrbL	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/0);  total (12/0)

AACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGG  >  W3110S.gb/3348893‑3348948                            |                             aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:1238695/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:1628950/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:1648865/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2086853/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2221489/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2324104/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2462260/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2649596/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2800116/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:2931777/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:3025817/1‑56 (MQ=255) aaCCTCGTGAGAGCAAAACGCTGGCCCACACTTCGCGCGAAGCTTAATCCAGCTgg  >  1:815081/1‑56 (MQ=255)                            |                             AACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGG  >  W3110S.gb/3348893‑3348948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑