Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,374,409	A→G	intergenic (‑65/‑315)	nanR ← / → dcuD	DNA‑binding transcriptional dual regulator/predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,374,409	0	A	G	100.0%	213.6 / NA	58	intergenic (‑65/‑315)	nanR/dcuD	DNA‑binding transcriptional dual regulator/predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/9);  total (49/9)

AAGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAAATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGA  >  W3110S.gb/3374350‑3374456                                                            |                                                aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:1091652/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:2469097/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:2426518/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:2843182/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:2271161/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:376076/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:485892/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:572013/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:1356915/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:1108524/1‑65 (MQ=255) aaGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGc                                            >  1:851199/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:630408/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:1003162/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:761220/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:1691389/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:1725682/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:1226221/1‑65 (MQ=255)               tAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:23475/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCttt                              >  1:1947710/1‑63 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:2844044/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:2577393/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:2778169/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:2360414/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:896862/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:2183772/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:136926/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:484130/1‑65 (MQ=255)                 aCATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTtgt                            >  1:1459346/1‑65 (MQ=255)                    tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                         <  1:2508596/65‑1 (MQ=255)                    tcatcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                         <  1:1410471/65‑1 (MQ=255)                     catcaGTGACATCCTATCACAGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGcc                         <  1:1218858/64‑1 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2440548/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:824457/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:1265130/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:660079/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:1629687/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:167091/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:475003/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:347769/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:3033518/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:3027706/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:3003933/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2034596/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2444495/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2314031/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2600360/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:26348/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2726479/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:2372107/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:27657/1‑65 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGCAGGGGTTTCTCTTTGTGCCTCATCATTACCATAAt         >  1:2945059/1‑63 (MQ=255)                                      cacaGGATTGAAAGTAGGGGAAGATGGAAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTa       >  1:917725/1‑65 (MQ=255)                                           gATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:173676/65‑1 (MQ=255)                                           gATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:1826897/65‑1 (MQ=255)                                           gATTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:879313/65‑1 (MQ=255)                                            aTTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:525708/64‑1 (MQ=255)                                            aTTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:2802812/64‑1 (MQ=255)                                            aTTGAAAGTAGGGGAAGATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGa  <  1:1189500/64‑1 (MQ=255)                                                            |                                                AAGGTCAGTGTGATTAACATCATCAGTGACATCCTATCACAGGATTGAAAGTAGGGGAAAATGGCAGGGTTTTCTCTTTGTGCCTCATCATTACCATAATTAACGGA  >  W3110S.gb/3374350‑3374456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑