Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,404,742	G→A	intergenic (+429/‑549)	yhdH → / → accB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,404,742	0	G	A	100.0%	47.1 / NA	17	intergenic (+429/‑549)	yhdH/accB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/1);  total (16/1)

TCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTC  >  W3110S.gb/3404698‑3404766                                             |                         tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt      >  1:566197/1‑65 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCt      >  1:33779/1‑65 (MQ=255)     tctaGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:2605349/4‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATgt               >  1:2582359/1‑52 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCt          >  1:2680827/1‑57 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:812958/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:1008536/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:546992/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:483034/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:2684034/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:2086013/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:198095/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:1851356/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:1837891/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  >  1:1723248/1‑65 (MQ=255)     tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTAACATGt   >  1:1687181/1‑64 (MQ=255)           cGATGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTc  <  1:633194/59‑1 (MQ=255)                                             |                         TCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTC  >  W3110S.gb/3404698‑3404766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑