Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,442,070 A→G T497A (ACG→GCG)  thiC → thiamin (pyrimidine moiety) biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,442,0700AG100.0% 36.0 / NA 11T497A (ACG→GCG) thiCthiamin (pyrimidine moiety) biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/9);  total (2/9)

TCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGG  >  W3110S.gb/3442013‑3442119
                                                         |                                                 
tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGcc                                               >  1:1840832/1‑62 (MQ=255)
tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGcc                                               >  1:516744/1‑62 (MQ=255)
 cGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaaaa                                           <  1:1428214/65‑1 (MQ=255)
                                          aTGCTCTTTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:1912680/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:1554160/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:1643125/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:2138907/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:2644072/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:2850410/65‑1 (MQ=255)
                                          aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:2856736/65‑1 (MQ=255)
                                           tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg  <  1:288458/64‑1 (MQ=255)
                                                         |                                                 
TCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGG  >  W3110S.gb/3442013‑3442119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: