Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,442,070 | A→G | T497A (ACG→GCG) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,442,070 | 0 | A | G | 100.0% | 36.0 / NA | 11 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (2/9); total (2/9) |
TCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGG > W3110S.gb/3442013‑3442119 | tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGcc > 1:1840832/1‑62 (MQ=255) tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGcc > 1:516744/1‑62 (MQ=255) cGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaaaa < 1:1428214/65‑1 (MQ=255) aTGCTCTTTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:1912680/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:1554160/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:1643125/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:2138907/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:2644072/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:2850410/65‑1 (MQ=255) aTGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:2856736/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAggg < 1:288458/64‑1 (MQ=255) | TCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGG > W3110S.gb/3442013‑3442119 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |