Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,473,895	A→G	E162G (GAG→GGG)	trmA →	tRNA (uracil‑5‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,473,895	0	A	G	100.0%	181.3 / NA	50	E162G (GAG→GGG)	trmA	tRNA (uracil‑5‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/14);  total (36/14)

CTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGT  >  W3110S.gb/3473837‑3473956                                                           |                                                              cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1122767/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1974396/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:2530781/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:2063869/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1664113/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:2092700/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1597386/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1549672/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg                                                          >  1:1266802/1‑64 (MQ=255) cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTg                                                           >  1:2123190/1‑63 (MQ=255)   gcgcgcgcAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATc                                                       >  1:1423222/1‑65 (MQ=255)                   aaTGTGCATCTGGTTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:3053521/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGTTGGATCAGGATTACATCGATGa                                       >  1:363097/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATg                                        >  1:1252688/1‑64 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2391918/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2390411/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:241961/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:718301/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2239056/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2458310/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:255005/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2995682/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:3031883/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:572154/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:665394/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:705831/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:1011081/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:1249363/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:141248/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2164609/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:2004807/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:1518322/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:219585/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:1660711/1‑65 (MQ=255)                   aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa                                       >  1:1617989/1‑65 (MQ=255)                                 tGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGt                           >  1:490960/1‑63 (MQ=255)                                           aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga               <  1:2291847/65‑1 (MQ=255)                                           aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga               <  1:1800053/65‑1 (MQ=255)                                           aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga               <  1:2482307/65‑1 (MQ=255)                                           aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga               <  1:3029725/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:2729391/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:1469415/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:342106/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:173945/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:176315/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:1784902/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:2144492/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:2307317/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt  <  1:2298903/65‑1 (MQ=255)                                                        tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGGCGCAGGGAAAGAGATGATCTACCGt  <  1:1146201/65‑1 (MQ=255)                                                           |                                                              CTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGT  >  W3110S.gb/3473837‑3473956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑