Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,497,705	T→G	R20R (CGT→CGG)	gldA →	glycerol dehydrogenase, NAD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,497,705	0	T	G	100.0%	71.6 / NA	21	R20R (CGT→CGG)	gldA	glycerol dehydrogenase, NAD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/0);  total (21/0)

AATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTG  >  W3110S.gb/3497662‑3497763                                            |                                                           aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:875533/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:1153166/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:816805/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:563982/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:557694/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:1981503/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:2084837/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:2216911/1‑51 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:2654912/1‑51 (MQ=255)  aTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCg                                                     >  1:2803903/1‑50 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:2329653/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:517169/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:2957660/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:2384549/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1089848/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1828134/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1708053/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1704557/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1350909/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTg  >  1:1093403/1‑65 (MQ=255)                                      tAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCCGGTTAGTGGTGGGTGACAAATTTg  >  1:2642614/1‑65 (MQ=255)                                            |                                                           AATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTG  >  W3110S.gb/3497662‑3497763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑