Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,500,231	C→T	A115T (GCG→ACG)	yijE ←	predicted permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,500,231	0	C	T	100.0%	109.9 / NA	33	A115T (GCG→ACG)	yijE	predicted permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/2);  total (31/2)

ATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCT  >  W3110S.gb/3500185‑3500281                                               |                                                   aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCaccacc                                    >  1:480407/1‑63 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCaccacc                                    >  1:410826/1‑63 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:2891818/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:823676/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:76103/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:147101/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:1698807/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:35223/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:2633354/1‑64 (MQ=255) aTCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCAccc                                   >  1:468487/1‑64 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:347104/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:3072850/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:2863656/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:403209/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:569153/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:2181816/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:1395078/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:1352767/1‑65 (MQ=255)     cgAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAAc                              >  1:1042289/1‑65 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:902638/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:2655519/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:2626589/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:2595792/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:2383472/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:219831/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:1395411/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:1297446/1‑61 (MQ=255)                ccACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATg                        >  1:715872/1‑60 (MQ=255)                caaCGTCGCAGGCGTTTACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATgg                       >  1:837665/3‑61 (MQ=39)                           gCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCg        <  1:420809/65‑1 (MQ=255)                                 aCCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCt  >  1:2309249/1‑65 (MQ=255)                                 aCCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCt  >  1:1427417/1‑65 (MQ=255)                                  ccGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCt  <  1:668917/64‑1 (MQ=255)                                               |                                                   ATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCTCAGGATCGCCACCT  >  W3110S.gb/3500185‑3500281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑