BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,572,896	C→T	R171* (CGA→TGA)	ompL →	predicted outer membrane porin L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,572,896	0	C	T	100.0%	495.4 / NA	145	R171* (CGA→TGA)	ompL	predicted outer membrane porin L
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (83/62); total (83/62)

Only 100 of 145 total aligned reads displayed.

CCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCT  >  W3110S.gb/3572834‑3572956

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:623859/1‑65 (MQ=255)

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:469004/1‑65 (MQ=255)

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:1997191/1‑65 (MQ=255)

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:2031531/1‑65 (MQ=255)

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:1155955/1‑65 (MQ=255)

ccTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGa                                                            >  1:2186943/1‑65 (MQ=255)

      ggAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAAt                                                      >  1:1756749/1‑65 (MQ=255)

      ggAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAAt                                                      >  1:59235/1‑65 (MQ=255)

      ggAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAAt                                                      >  1:895251/1‑65 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:216808/65‑1 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:2109658/65‑1 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:797488/65‑1 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:458513/65‑1 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:2535549/65‑1 (MQ=255)

          ttttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:640232/65‑1 (MQ=255)

           tttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:1866134/64‑1 (MQ=255)

           tttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:1688400/64‑1 (MQ=255)

           tttAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACt                                                  <  1:1654086/64‑1 (MQ=255)

                 aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                           <  1:305157/65‑1 (MQ=255)

                 aTCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                           <  1:1421784/65‑1 (MQ=255)

                  tCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                           <  1:1778626/64‑1 (MQ=255)

                  tCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAAtag                                           <  1:566135/64‑1 (MQ=255)

                                 ccTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGa                            <  1:929215/64‑1 (MQ=255)

                                 ccTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGa                            <  1:2377790/64‑1 (MQ=255)

                                 ccTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGa                            <  1:1801040/64‑1 (MQ=255)

                                 ccTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGa                            <  1:3001830/64‑1 (MQ=255)

                                  cTATACATTTGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGat                           <  1:1560869/64‑1 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTggg                 <  1:1433162/65‑1 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTggg                 <  1:892587/65‑1 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:285373/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:1060920/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2723581/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2929811/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2338749/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2628531/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:397767/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:1226529/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:1236614/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2538220/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:719959/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:2042812/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:711248/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:664293/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:660118/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:1489570/1‑64 (MQ=255)

                                           tGAGCCACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:1544616/1‑64 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTgg                  >  1:686182/1‑60 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa               >  1:750044/1‑63 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:930309/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2620197/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2680890/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2618989/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2924108/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2968000/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:317255/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:348266/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:436537/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:454172/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:5081/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:259288/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:1645532/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2011137/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:137419/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2251894/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:1323065/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:1284553/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:1197482/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2290134/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2309214/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2317044/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:1148158/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:1084166/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:23729/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:1060626/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:238844/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2442369/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2486040/1‑65 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2519929/65‑1 (MQ=255)

                                               ccACATTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             >  1:2527716/1‑65 (MQ=255)

                                                cacaTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAAt             <  1:2156345/64‑1 (MQ=255)

                                                   aTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt         <  1:1364289/65‑1 (MQ=255)

                                                   aTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt         <  1:157095/65‑1 (MQ=255)

                                                   aTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt         <  1:104877/65‑1 (MQ=255)

                                                   aTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt         <  1:1723766/65‑1 (MQ=255)

                                                   aTTACTTCATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACt         <  1:1768645/65‑1 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGaaa                              >  1:647927/1‑37 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGaa               >  1:2244776/1‑52 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:1867083/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:1888747/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:1623388/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:46192/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:2206241/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:2284978/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:3099534/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:3063697/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:294462/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:1129804/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:2343356/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:2856039/1‑65 (MQ=255)

                                                          cATGTGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCt  >  1:2577876/1‑65 (MQ=255)

CCTACTGGAATTTTAAAATCACCGATAAATTTTCCTATACATTTGAGCCACATTACTTCATGCGAGTGAATGACTTTAATAGTAGCAACGGGAAAGATCATCATTGGGAAATCACTAACACCT  >  W3110S.gb/3572834‑3572956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`