Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,582,634	A→G	D411G (GAC→GGC)	glnG →	fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,582,634	0	A	G	100.0%	81.9 / NA	23	D411G (GAC→GGC)	glnG	fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

TCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAG  >  W3110S.gb/3582585‑3582680                                                  |                                               tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:759888/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:1589719/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:1809065/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:1935549/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:2102280/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:684323/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:2173494/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:45766/1‑65 (MQ=255) tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc                                 >  1:317029/1‑65 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:2190299/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:999240/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:754898/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:51556/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:2685279/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:2656457/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:2563165/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:221129/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:1164889/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:218939/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:2165186/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:1448363/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:1395529/65‑1 (MQ=255)                                ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg  <  1:1295907/65‑1 (MQ=255)                                                  |                                               TCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAG  >  W3110S.gb/3582585‑3582680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑