Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,587,844	T→C	P624P (CCA→CCG)	polA ←	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,587,844	0	T	C	100.0%	110.4 / NA	30	P624P (CCA→CCG)	polA	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/11);  total (19/11)

GATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACG  >  W3110S.gb/3587803‑3587890                                          |                                               gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:813701/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:1533060/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:1557761/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:1656564/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:557882/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:3092922/65‑1 (MQ=255) gATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAg                         <  1:3058904/65‑1 (MQ=255)    ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAgaaa                      >  1:1615764/1‑62 (MQ=255)    ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAgaaa                      >  1:1695228/1‑62 (MQ=255)    ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAgaaa                      >  1:202326/1‑62 (MQ=255)    ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAgaaa                      >  1:252919/1‑62 (MQ=255)    ttCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAgaa                       >  1:3051028/1‑62 (MQ=255)        gCTTCGCCAGCCCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:1429872/1‑65 (MQ=255)        gCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:771533/1‑65 (MQ=255)        gCTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2328398/1‑65 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGc                                             >  1:973210/1‑37 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2745037/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2804683/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2810948/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2938902/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:3001161/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:248141/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:2364631/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:1792324/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:174187/1‑64 (MQ=255)         cTTCGCCAGACCACGATACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTcc                  >  1:1468020/1‑64 (MQ=255)                        aTACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACg  <  1:2794212/65‑1 (MQ=255)                        aTACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACg  <  1:592829/65‑1 (MQ=255)                        aTACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACg  <  1:1429484/65‑1 (MQ=255)                        aTACTCCAGAATCACTTTCGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACg  <  1:1314116/65‑1 (MQ=255)                                          |                                               GATTTCAGCTTCGCCAGACCACGATACTCCAGAATCACTTTTGGCAACGGATAGTCCAGCGCCAGTTCTTCCAGTACCTCTTCCGACG  >  W3110S.gb/3587803‑3587890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑