Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,594,183	A→G	D28D (GAT→GAC)	yihE ←	predicted kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,594,183	0	A	G	100.0%	153.5 / NA	41	D28D (GAT→GAC)	yihE	predicted kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/30);  total (11/30)

TCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAATCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTG  >  W3110S.gb/3594130‑3594242                                                      |                                                            tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2818225/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:144508/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:92048/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2882398/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:3055855/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:1684722/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2562750/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:55817/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:442496/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2088740/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2174272/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:2230712/63‑1 (MQ=255) tCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:237706/63‑1 (MQ=255)                      ttttCATAGCTGTTAAGCGGGGTAAGACCGGAGTCCACCCgg                                                    <  1:573204/42‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCTATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:137787/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:517202/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:3095109/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:627266/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:861266/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:904103/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:1430408/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:2857748/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:2652765/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:2567701/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:1554887/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:1622452/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:1972967/64‑1 (MQ=255)                                      gCGGGGTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCgg              <  1:1807548/64‑1 (MQ=255)                                         gggTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGgtgt          <  1:1904836/65‑1 (MQ=255)                                          ggTAAGACCGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGgtgt          <  1:608325/64‑1 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCg                               >  1:287746/1‑35 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGgt            >  1:2839157/1‑54 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTTTAGTGTCTg  >  1:2888785/1‑64 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTc    >  1:2987823/1‑62 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTc    >  1:445952/1‑62 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCt   >  1:940102/1‑63 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTg  >  1:1868928/1‑64 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTg  >  1:1631923/1‑64 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTg  >  1:481979/1‑64 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTg  >  1:2382430/1‑64 (MQ=255)                                                  cGGAGTCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTg  >  1:2529393/1‑64 (MQ=255)                                                      |                                                            TCCTGAAATTGATAGACACGGTTTTCATAGCTGTTAAGCGGGGTAAGACCGGAATCCACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTG  >  W3110S.gb/3594130‑3594242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑