Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,614,917	G→A	G513G (GGC→GGT)	ubiB ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,614,917	0	G	A	100.0%	233.6 / NA	71	G513G (GGC→GGT)	ubiB	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/29);  total (42/29)

ACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGT  >  W3110S.gb/3614857‑3614965                                                             |                                                 accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:208318/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:598104/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:1293559/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:2031420/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:1402005/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:2246892/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:860712/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:3035793/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:3007536/65‑1 (MQ=255) accTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCCGACCAACAAGAATGTACCAc                                              <  1:2548906/65‑1 (MQ=255)  ccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc                                              <  1:2835778/64‑1 (MQ=255)                              cccATTCAGGTCGGCTGACCAACAAGAATGTACCa                                               >  1:2476974/1‑35 (MQ=255)                              cccATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCgag                  >  1:222416/1‑64 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAActcg       >  1:2223861/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2500172/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:255152/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2557275/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:25372/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:834149/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:838629/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:786611/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2675563/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2731786/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2743418/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2885813/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2919480/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2945647/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:337976/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:832100/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:830140/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:822256/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:452793/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:55858/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:807176/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1058433/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1524867/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1587833/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1505202/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1444918/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1866631/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2495346/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1936231/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1375277/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2057599/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1141778/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:210928/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2146798/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2191686/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2239456/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:1087411/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg       >  1:2419650/1‑65 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc        >  1:1933638/1‑64 (MQ=255)                                        tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc        >  1:1972486/1‑64 (MQ=255)                                             tGACCATCAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:199458/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:966846/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:898895/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:856643/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:839449/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:985609/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:619479/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:422278/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:41469/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:352120/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:2635512/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:2395942/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:2064367/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:1852209/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:169446/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:1612482/65‑1 (MQ=255)                                             tGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:1250558/65‑1 (MQ=255)                                               aCCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGt  <  1:2501708/63‑1 (MQ=255)                                                             |                                                 ACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGT  >  W3110S.gb/3614857‑3614965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑